Metabolism of Inorganic Pyrophosphate

Metabolism of Inorganic Pyrophosphate. I. Microsomal Inorganic Pyrophosphate Phosphotransferase of Rat Liver.

The formation of large amounts of inorganic pyrophosphate as a by-product of numerous synthetic reactions in nearly all living tissues makes the metabolic reactions of this compound of interest. Rapid hydrolysis to orthophosphate is presumed to be the fate of most inorganic pyrophosphate but the possibility of the conservation and utilization of some of the energy of this compound has been a su...

Pathogenesis of chondrocalcinosis and pseudogout. Metabolism of inorganic pyrophosphate and production of calcium pyrophosphate dihydrate crystals.

Calcium pyrophosphate dihydrate (CPPD) is one of several types of crystal that may be deposited in the body. The term chondrocalcinosis is used to describe the radiographic appearance of calcified deposits, notably in the articular cartilage and menisci of the knees but also in other joints.' Not all calcified deposits within joints are due to calcium pyrophosphate; however, occasional depositi...

Understanding inorganic pyrophosphate metabolism: toward prevention of calcium pyrophosphate dihydrate crystal deposition.

A colorimetric determination of inorganic pyrophosphate.

During recent work on the mechanism of the ATP-CoA-acetate1 reaction (1, 2) we observed that, on incubation of yeast enzyme with ATP, CoA, acetate, and hydroxylamine, a substance was formed in the presence of fluoride which, although initially colorless with the Fiske-Subbarow phosphate reagents (3), slowly developed on standing a blue color of a somewhat more purplish shade than that obtained ...

Inorganic pyrophosphate generation and disposition in pathophysiology.

Inorganic pyrophosphate (PP(i)) regulates certain intracellular functions and extracellular crystal deposition. PP(i) is produced, degraded, and transported by specialized mechanisms. Moreover, dysregulated cellular PP(i) production, degradation, and transport all have been associated with disease, and PP(i) appears to directly mediate specific disease manifestations. In addition, natural and s...